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目的:筛选与精子活力相关的基因。方法:将活力正常者与弱精子症者精子cDNA与Affymetrix全基因组芯片进行杂交,筛选出差异表达基因。RT-PCR分析该基因在人体不同组织及精子中的表达,并比较该基因在活力正常和活力低下的人精子中表达的差异。结果:芯片结果分析筛选出差异表达基因:电压依赖性阴离子通道(VDACs)基因。VDACs包括VDAC1、VDAC2、VDAC33个亚型,它们均在人精子中表达。与活力正常组精子VDAC2相对表达量(0.803±0.043)比较,VDAC2在弱精子症组精子的相对表达量(0.568±0.036)显著降低(P<0.01)。结论:VDAC2的表达减少可能与精子中活力降低有关。
Objective: To screen the genes related to sperm motility. Methods: The sperm cDNA of sperm motility and asthenospermia were hybridized with Affymetrix whole genome microarray, and the differentially expressed genes were screened out. RT-PCR analysis of the gene expression in different tissues of human and sperm, and compared the gene expression in human normal and low motility sperm differences. Results: The results of the chip screening differentially expressed genes: voltage-dependent anion channels (VDACs) gene. VDACs include VDAC1, VDAC2, and VDAC33 subtypes, all of which are expressed in human spermatozoa. Compared with normal VDAC2 expression (0.803 ± 0.043), the relative expression of VDAC2 in spermatogenic spermatozoa (0.568 ± 0.036) was significantly decreased (P <0.01). Conclusion: The decreased expression of VDAC2 may be related to the decreased sperm motility.