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目的 研究和探讨微囊藻毒素 (MCLR)对动物的短期毒效应作用。方法 SD大鼠经腹腔注射不同剂量的MCLR ,分别于注射的 1、7d和停药后第 7天 (即第 14天 )采集大鼠的血清和肝、肾、心等组织标本 ,经酶学及病理学检验 ,观察其损伤效应。结果 12 2 μg/kg剂量组 ,注射后 2 4h可观察到大鼠心肌细胞肌浆溶解 ,核变性固缩 ,肌原纤维局部坏死 ;血清中天冬氨酸转氨酶 (AST)、乳酸脱氢酶 (LDH)和磷酸肌酸激酶 (CPK)与其他组相比显著升高 ;肾细胞变性 ,血清中肌酐 (BCr )和尿素氮(BUN )亦显著升高 ;同时肝细胞片状出血、坏死 ,注射剂量为 5 0 μg/kg和 2 5 μg/kg时 ,仍出现肝细胞重度和轻度颗粒变性。血清中丙氨酸转氨酶 (ALT)、碱性磷酸酶 (LDH)和AST显著上升。对照组大鼠的肝、肾、心等脏器均正常。结论 MCLR可引起SD大鼠肝、肾、心等脏器的短期毒效应 ,且随着剂量的增加 ,损伤效应加重。
Objective To study and investigate the short-term toxic effect of microcystin (MCLR) on animals. Methods SD rats were injected intraperitoneally with different doses of MCLR, and the serums, liver, kidney and heart tissues of rats were collected on day 1, day 7 and day 7, respectively, And pathological examination to observe the damage effect. Results In the 12 μg / kg dose group, sarcomere, nuclear degeneration, and myofibrillar necrosis were observed in rat cardiac myocytes at 24 hrs after injection. Aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were significantly increased compared with the other groups. Renal cell degeneration, serum creatinine (BCr) and urea nitrogen (BUN) were also significantly increased. Hypertrophic and mild granulocytosis of hepatocytes still occurred at injection doses of 50 μg / kg and 25 μg / kg. Serum alanine aminotransferase (ALT), alkaline phosphatase (LDH) and AST increased significantly. Control rats liver, kidney, heart and other organs were normal. Conclusion MCLR can cause short-term toxic effects of liver, kidney, heart and other organs in SD rats, and the damage effect is aggravated with the increase of dose.