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[目的]观察加味四君子汤治疗对N-甲基-N-硝基-亚硝基胍(MNNG)诱发大鼠胃癌的预防作用。[方法]90只雄性Wister大鼠随机分成5组,正常对照组,模型组,加味四君子汤组,增液汤组,0.85%氯化钠组。每组18只,除正常对照组外,其余各组MNNG造模。喂养(28±2)周后观察各组大鼠的胃黏膜病变。[结果]83只(92.2%)大鼠完成实验。模型组大鼠胃癌发生率40.0%(6/15),显著高于加味四君子汤组5.9%(1/17),差异有统计学意义(P<0.05),前者胃黏膜萎缩、肠上皮化生和异型增生发生率也显著高于后者(P<0.05)。增液汤组及0.85%氯化钠组与模型组大鼠胃癌及癌前病变发生率无明显差异(P>0.05);正常对照组无胃癌发生。[结论]脾虚血瘀是胃癌及癌前病变发生发展过程中的重要促进因素之一,加味四君子汤健脾化瘀治疗能有效地抑制MNNG诱发大鼠胃癌及癌前病变的发生。
[Objective] To observe the preventive effect of Modified Sijunzi Decoction on N-methyl-N-nitro-nitrosoguanidine (MNNG)-induced gastric cancer in rats. [Methods] Ninety male Wister rats were randomly divided into 5 groups: normal control group, model group, Jiawei Sijunzi Decoction group, Zengye Decoction group, 0.85% sodium chloride group. In each group of 18, except for the normal control group, the remaining groups of MNNG were modeled. After 28 (2 ± 2) weeks of feeding, gastric mucosal lesions were observed in each group. [Results] Eighty-three (92.2%) rats completed the experiment. The incidence rate of gastric cancer in the model group was 40.0% (6/15), which was significantly higher than 5.9% (1/17) in the Modified Sijunzi Decoction group. The difference was statistically significant (P<0.05). The former had gastric mucosal atrophy and intestinal epithelial metaplasia. The incidence of dysplasia was also significantly higher than that of the latter (P < 0.05). There was no significant difference in the incidence of gastric cancer and precancerous lesions between the Zengyetang group and the 0.85% sodium chloride group and the model group (P>0.05). No gastric cancer occurred in the normal control group. [Conclusion] Spleen deficiency and blood stasis is one of the important factors in the development of gastric cancer and precancerous lesions. Jiawei Sijunzi Decoction can effectively inhibit the occurrence of gastric cancer and precancerous lesions in rats induced by MNNG.