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目的:探讨活血化瘀方药血塞通胶囊调控造血干细胞(hemopoietic stem cell,HSC)“生新血”的机制。方法:参照改良Zea-Longa法建立大鼠大脑中动脉脑缺血再灌注损伤模型(middle cerebral artery occlusion,MCAO)。随机分为正常组、模型组、血塞通高、中、低剂量组;各组再随机按1,3,7,14,28 d时间点分为亚组。血塞通胶囊配置成20,40,60 g·L-1灌胃。正常组和模型组生理盐水灌胃,每天1次,直到实验结束。ELISA法检测不同时间点血液和骨髓中干细胞因子(stem cell factor,SCF)表达情况;流式细胞仪检测外周血和骨髓中CD117的变化。结果:模型组外周血和骨髓中CD117+HSC和SCF从1 d开始升高,14 d到高峰,其后逐渐降低。血塞通高、中剂量组外周血和骨髓中CD117+HSC和SCF在同一时间点上升的趋势明显优于模型组(P<0.05)。结论:活血化瘀生新层面之“生新血”是通过调控CD117+HSC数量变化实现生新血目的。
Objective: To investigate the mechanism of Xuesaitong Capsule on regulating hemopoietic stem cells (HSC). Methods: A middle cerebral artery occlusion (MCAO) model of middle cerebral artery was established according to the modified Zea-Longa method. Randomly divided into normal group, model group, Xuesaitong high, medium and low dose groups; each group was randomly divided into subgroups at 1,3,7,14,28 d. Xuesaitong capsule configured to 20,40,60 g · L-1 gavage. The normal group and model group were given normal saline once a day until the experiment was over. ELISA was used to detect the expression of stem cell factor (SCF) in blood and bone marrow at different time points. The changes of CD117 in peripheral blood and bone marrow were detected by flow cytometry. Results: CD117 + HSC and SCF in peripheral blood and bone marrow of model group increased from 1 d to 14 d, then decreased gradually. Compared with the model group, the levels of CD117 + HSC and SCF in Xuesaitong high and medium dose groups increased significantly at the same time point (P <0.05). Conclusion: The new blood level of blood circulation for promoting blood circulation and blood circulation is to achieve the goal of new blood circulation by regulating the number of CD117 + HSCs.