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应用mRNA差异显示技术,采用引物T12MA和任意引物A,发现在自发性高血压大鼠(SHR)和正常对照大鼠(WKY)血管平滑肌之间mRNA转录有明显不同;已筛选出数种特异的SHR和WKYcDNA克隆,并进行序列分析。在大鼠最新基因文库中,所筛选出的这几个cDNA克隆无同源性。
Using mRNA differential display technique, mRNA transcription was found to be significantly different between vascular smooth muscle in spontaneously hypertensive rats (SHR) and normal control rats (WKY) using primers T12MA and any primer A; several specific SHR and WKY cDNAs were cloned and sequenced. In the latest rat gene library, the selected cDNA clones have no homology.