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采用核糖体DNA内转录间隔区(nrDNA ITS)序列比较分析了甘薯及其近缘野生种的遗传多样性及系统进化关系,首次报道了栽培种甘薯‘徐薯18’(Ipomoea batatas‘Xushu18’)及其近缘野生种I.triloba(DOM),I.cordatotriloba(MEX),I.nil(PER),I.nil(JPN),I.hederacea Jacq.(USA),I.hederacea Jacq.(HK)和种间杂交种67-1(I.batatas‘Xushu18’×I.hederacea Jacq.)及回交种(67-1×I.batatas‘Xushu18’)的nrDNA ITS序列。序列分析表明,栽培种甘薯及其近缘野生种nrDNA ITS序列长度为570~600bp。其中,ITS1序列为185~209 bp,GC含量为53.11%~61.83%;ITS2序列为214~226 bp,GC含量为61.21%~72.89%;5.8S序列均为165 bp,GC含量为54.55%~55.76%。此外,栽培种甘薯及其近缘野生种ITS序列信息位点均集中在ITS1和ITS2区;与其他甘薯属植物相比,I.wrightii ITS2的末端缺失了6~8个碱基。系统进化分析表明,栽培种甘薯‘徐薯18’(I.batatas‘Xushu18’)和野生种I.triloba、I.cordatotriloba、I.lacunosa、I.trifida的亲缘关系较近,与I.wrightii、I.pes-tigridis、I.grandifolia、I.nil、I.hederacea Jacq.、I.purpurea的亲缘关系较远;杂交后代与栽培种甘薯‘徐薯18’(I.batatas‘Xushu18’)亲缘关系较近,与野生种父本I.hederacea Jacq.的亲缘关系较远。
The genetic diversity and phylogenetic relationship of sweet potato and its wild relatives were analyzed using the sequence of nrDNA ITS in ribosomal DNA. It was first reported that cultivars of Ipomoea batatas ’Xushu 18’ And its related wild species I.triloba (DOM), I.cordatotriloba (MEX), I.nil (PER), I.nil (JPN), I.hederacea Jacq. (USA), I. hederacea Jacq. ) And nrDNA ITS sequences of I.batatas’Xushu18 ’× I. hederacea Jacq. And backcross (67-1 × I.batatas’Xushu18’). Sequence analysis showed that the length of nrDNA ITS sequence of cultivated sweet potato and its related wild species was 570-600 bp. The ITS1 sequence ranged from 185 to 209 bp and the GC content ranged from 53.11% to 61.83%. The ITS2 sequence ranged from 214 to 226 bp with a GC content of 61.21% to 72.89%. The 5.8S sequence was 165 bp with a GC content of 54.55% 55.76%. In addition, ITS sequence information sites of cultivated sweet potato and its related wild species were concentrated in ITS1 and ITS2 regions. Compared with other Ipomoea plants, the terminal of I.wrightii ITS2 was missing 6-8 bases. Phylogenetic analysis showed that the genetic relationship between cultivated sweetpotato ’Batatatas’Xushu 18’ and the wild species I.triloba, I.cordatotriloba, I.lacunosa, I.trifida was close, and that of I.wrightii, I.pes-tigridis, I.grandifolia, I.nil, I.hederacea Jacq., I.purpurea were distantly related. The genetic relationship between hybrid progeny and cultivated sweet potato ’Xushu 18’ (I.batatas’Xushu18 ’) Closer, more distantly related to wild herpetotypic I. hederacea Jacq.