论文部分内容阅读
目的：复制一种创伤后肠源性脓毒症和多脏器功能失常综合征（ＭＯＤＳ）动物模型。方法：家兔饲入大肠杆菌（Ｏ１１１Ｂ４）１．５×１０１１个／ｋｇ后，造成低血容量性休克〔平均动脉压为５．３ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ），维持１小时〕和再灌注损伤，术后静脉输入５％葡萄糖生理盐水５０ｍｌ·ｋｇ－１／ｄ，实验观察１４日。另设５个对照组，分别观察手术、饲菌、休克、复苏和内毒素对器官功能的影响。结果：实验组动物休克复苏后１日～３日出现肠源性菌血症和内毒素血症，随后表现出全身炎症反应、高血糖和氨基酸代谢障碍。实验组动物死亡率和ＭＯＤＳ发生率分别为８８．３％和７１．６％。病理学观察显示，肝、肾、肺和小肠多脏器实质性损害。结论：本模型模拟了创伤后ＭＯＤＳ的常见诱因和临床特征，ＭＯＤＳ发病率高达７０％以上，是较理想的创伤后单相速发型ＭＯＤＳ的动物模型
PURPOSE: To duplicate an animal model of post-traumatic gut-derived sepsis and multiple organ dysfunction syndrome (MODS). METHODS: Rabbits were challenged with 1.5 × 10 11 / kg E. coli (O111B4), resulting in hypovolemic shock (mean arterial pressure of 5.3 kPa (1 kPa = 7.5 mmHg) for 1 hour) and reperfusion Injury, postoperative intravenous infusion of 5% glucose saline 50ml · kg-1 / d, the experimental observation of 14 days. The other 5 control groups were observed surgery, feeding bacteria, shock, resuscitation and endotoxin on organ function. Results: The intestinal bacteremia and endotoxemia occurred on the 1st to the 3rd day after shock in experimental group, followed by systemic inflammatory response, hyperglycemia and amino acid metabolism. Animal mortality and MODS incidence in the experimental group were 88.3% and 71.6% respectively. Pathological observations showed substantial damage to the liver, kidney, lung and small intestine. Conclusion: This model simulates the common causes and clinical features of MODS after trauma. The morbidity of MODS is over 70%, which is an ideal animal model of MODS after trauma