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用吸附法将甲烷菌整细胞固定在某些有机和无机载体上,制备了丙烯环氧化反应的固定化生物催化剂。不同载体对固定化细胞的催化活性有很大影响,用一填充床流动反应器对吸附在不同载体上的固定化细胞进行了活性考察。结果表明:用有机的疏水载体如聚丙烯,石蜡等制成的固定化细胞具有较高的环氧化催化活性,且不同载体制成的固定化细胞的催化活性大致相同,约在28nmol/mg cell。用无机亲水类载体制成的固定化细胞,其环氧化催化活性差别很大,且大多数这类载体均比有机的疏水载体活性低,但用特殊方法处理过的砂子为载体制成的固定化细胞,其催化活性很高。还发现,用3AK型分子筛和酸处理过的Al_2O_3和3AK型分子筛为载体时,固定化细胞的活性与载体表面酸性有一定关系。
The immobilized biocatalysts for the epoxidation of propylene were prepared by immobilizing methanocyte whole cells on some organic and inorganic supports by adsorption method. Different carriers have a great influence on the catalytic activity of immobilized cells. The activity of immobilized cells adsorbed on different carriers was investigated by a packed bed flow reactor. The results showed that the immobilized cells made of organic hydrophobic carriers such as polypropylene and paraffin have higher epoxidation catalytic activity, and the catalytic activity of immobilized cells made by different carriers is about the same, about 28nmol / mg cell. Immobilized cells made of an inorganic hydrophilic carrier have a very different epoxidation catalytic activity and most of these carriers are less active than organic hydrophobic carriers but are prepared using specially treated sand as a carrier Immobilized cells, its catalytic activity is high. It was also found that the immobilized cells activity was related to the surface acidity of the carrier when zeolite A 3AK and acid-treated Al 2 O 3 and 3AK zeolites were used as carriers.