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目的 探讨血清miR-214在肾透明细胞癌患者中的表达及在诊断、治疗及预后判断方面的临床意义.方法 选择我院确诊的肾透明细胞癌30例(肾癌组),选取同期在我院体检健康的30例(对照组).对照组随机检测血清miR-214水平,肾癌组于术前、术后1周及术后1个月检测血清miR-214变化,比较组间的表达差异及其与临床参数的关系.结果 肾癌组术前、术后1周与对照组血清miRNA-214相对表达量比较差异有统计学意义(P<0.05);肾癌组术前与术后1个月、术后1周与术后1个月之间比较差异均有统计学意义(P<0.05);肾癌组术前与术后1周、术后1个月与对照组之间比较差异无统计学意义(P=0.318,P=0.383).利用受试者工作特征曲线做鉴别检验,获得曲线下面积(AUC)=0.858[95%CI(0.756,0.960),P<0.05],根据Youden指数找出最佳诊断点为2.91,敏感度为80.0%,特异度为90.0%.结论 血清miR-214与肾透明细胞癌有明显相关性,可作为该病的潜在生物标记物.“,”Objective To investigate expression of serum miR-214 in patients with clear cell carcinoma of kidney and its clinical significance in diagnosis,therapy and prognosis judgement.Methods A total of 30 patients with clear cell carcinoma of kidney were selected as renal cancer group,and 30 healthy people taking medical examination at the same period were chosen as control group,and miR-214 expressions were detected by randomized method in control group,and the expressions were detected before surgery,1 week and 1 month after surgery in renal cancer group,and then expression differences and correlations with clinical indexes were compared between two groups.Results The differences of relative miR-214 expressions before and 1 week after surgery in renal cancer group and in control group were statistically significant (P<0.05).The differences of miR-214 expressions were statistically significant between before surgery and 1 month after surgery,and between 1 week after surgery and 1 month after surgery in renal cancer group (P<0.05).The differences of miR-214 expressions were not statistically significant between before and 1 week after surgery in renal cancer group,and between 1 month after surgery in renal cancer group with in control group (P=0.318,P=0.383).Diagnostic test was performed by using receiver operating curve (ROC),and area under curve (AUC) was 0.858 [95%CI (0.756,0.960),P<0.05].The best diagnostic point,sensitivity and specificity were 2.91,80.0% and 90.0% respectively according to Youden index.Conclusion Expressions of serum miR-214 in patients with clear cell carcinoma of kidney have correlation with clear cell carcinoma of kidney,and therefore it can be used as a potential molecular marker.