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观察了血管紧张素Ⅱ（ＡｎｇⅡ）的１型（ＡＴ１）和２型（ＡＴ２）受体及白细胞介素－１β转化酶（ＩＣＥ）在人胎肾系膜细胞表达情况及其与肾小球系膜细胞凋亡的关系。采用６个月龄人胎肾培养肾小球系膜细胞，经１０－８、１０－７、１０－６和１０－５ｍｏｌ／Ｌ浓度的ＡｎｇⅡ诱导后，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术检测了胎肾系膜细胞的ＡＴ１、ＡＴ２受体和ＩＣＥ基因的ｍＲＮＡ表达情况。经ＡｎｇⅡ刺激后ＡＴ１受体ｍＲＮＡ表达有明显增强，并呈浓度依赖性，而ＡＴ２受体和ＩＣＥ基因的ｍＲＮＡ表达仅在１０－５ｍｏｌ／Ｌ的ＡｎｇⅡ刺激后才检测到。用１０－５ｍｏｌ／Ｌ浓度的ＡｎｇⅡ诱导系膜细胞凋亡，分别于２４、４８和７２ｈ收集细胞进行检测，应用碘化丙啶染色（ＰＩ）、原位末端标记染色（ＴＵＮＥＬ）、染色质ＤＮＡ电泳和流式细胞仪等定性和定量方法观察系膜细胞的凋亡改变。经１０－５ｍｏｌ／Ｌ浓度的ＡｎｇⅡ刺激７２ｈ后，肾小球系膜细胞出现了典型的凋亡征象，包括细胞核固缩和碎裂，染色质ＤＮＡ片段化，流式细胞仪检测到标志着细胞凋亡的Ａｏ峰；应用ＡＴ１受体抗体封闭后对这种凋亡过程无显著影响。结果表明，ＡｎｇⅡ诱导的系膜细胞凋亡可能是通过其ＡＴ２受?
The expression of angiotensin Ⅱ type 1 (AT1) and type 2 (AT2) receptors and interleukin-1β converting enzyme (ICE) in human fetal renal mesangial cells and their relationship with glomerular Membrane cell apoptosis. Glomerular mesangial cells were cultured in 6-month-old human fetus kidneys and induced by AngⅡ at the concentrations of 10-8, 10-7, 10-6 and 10-5mol / L, and then analyzed by reverse transcription-polymerase chain reaction -PCR) technique was used to detect mRNA expression of AT1, AT2 receptor and ICE gene in fetal kidney mesangial cells. The expression of AT1 receptor mRNA was significantly increased in a concentration-dependent manner after AngⅡ stimulation, while the mRNA expression of AT2 receptor and ICE mRNA was detected only after stimulation with 10-5mol / L AngII. The apoptosis of mesangial cells was induced by Ang Ⅱ at a concentration of 10-5mol / L, and the cells were harvested at 24, 48 and 72 hours respectively. Propidium iodide (PI), TUNEL, Electrophoresis and flow cytometry and other qualitative and quantitative methods to observe the apoptosis of mesangial cells. After stimulation with 10-5mol / L AngⅡ for 72h, typical mesangial cells showed signs of apoptosis, including pyknosis and fragmentation of nuclei, fragmentation of chromatin, identification of cells by flow cytometry Ao peak of apoptosis; blocking with AT1 receptor antibody had no significant effect on this apoptosis process. The results show that, Ang Ⅱ induced mesangial cell apoptosis may be affected by its AT2?