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目的探讨血脂及载脂蛋白E(ApoE)基因多态性与结肠腺瘤的相关性,方法对98例结肠腺瘤患者和40例正常对照者,酶法测定血浆脂质水平,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法测定 ApoE 基因型。差异性分析采用 X~2和 t 检验。结果结肠腺瘤组、远侧结肠腺瘤组和绒毛状腺瘤组血浆胆固醇水平分别为(5.32±0.85)、(5.58±0.63)和(5.49±0.69)mmol/L,高于对照组、近侧结肠腺瘤组和非绒毛状腺瘤组的(4.28±0.62)、(4.82±0.58)和(4.76±0.58)mmol/L(P<0.05);而结肠腺瘤组血浆高密度脂蛋白胆固醇水平为(1.39±0.25)mmol/L,低于对照组的(1.51±0.29)mmol/L(P<0.05);结肠腺瘤组各基因型血脂水平差异无统计学意义;结肠腺瘤组 ApoEε3/ε4基因型频率(7.1%)低于对照组(17.5%,P<0.05)。结论血浆胆固醇水平增高者结肠腺瘤发生的危险性增加,ApoEeε/ε4基因型携带者其结肠腺瘤发生的危险性减少。
Objective To investigate the relationship between plasma lipoprotein and apolipoprotein E (ApoE) gene polymorphism and colorectal adenoma.Methods 98 patients with colonic adenoma and 40 normal controls were enrolled in this study. Enzymatic determination of plasma lipid levels, polymerase chain reaction ApoE genotypes were determined by restriction fragment length polymorphism (PCR-RFLP). Difference analysis using X ~ 2 and t test. Results The plasma levels of cholesterol in the colon adenoma group, distal colorectal adenoma group and villous adenoma group were (5.32 ± 0.85), (5.58 ± 0.63) and (5.49 ± 0.69) mmol / L, respectively, which were higher than those in the control group (4.28 ± 0.62), (4.82 ± 0.58) and (4.76 ± 0.58) mmol / L, respectively (P <0.05) in colorectal adenoma group and non-villous adenoma group, while those in colorectal adenoma group were significantly higher than those in colorectal adenoma group (1.39 ± 0.25) mmol / L, which was lower than that of the control group (1.51 ± 0.29) mmol / L (P <0.05). There was no significant difference in the levels of serum lipids between the colon adenomas and the colon adenomas / ε4 genotype frequency (7.1%) lower than the control group (17.5%, P <0.05). Conclusions The increased risk of colorectal adenoma with elevated plasma cholesterol levels decreases the risk of developing colonic adenomas in ApoEeε / ε4 genotypes.