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本文研究了雷帕霉素（ｒａｐａｍｙｃｉｎ，ＲＰＭ）对大鼠佐剂性关节炎的作用，证明ＲＰＭ１０～５０ｍｇ／ｋｇ腹腔注射（ｉｐ）能完全预防弗氏完全佐剂（ＦＣＡ）所致大鼠关节继发肿胀的发生，对ＦＣＡ所致大鼠急性期原发性肿胀，环孢菌素Ａ（ＣｓＡ）效果优于ＲＰＭ，但ＣｓＡ停药不久即发生反跳。对继发肿胀的关节，ＲＰＭ５０ｍｇ／ｋｇｉｐ也显示了良好的治疗作用，且不发生反跳。Ｌ９２９细胞结晶紫染色法测得：关节炎大鼠腹腔巨噬细胞（ＭΦ）产生的肿瘤坏死因子α（ＴＮＦα）浓度显著升高，ＲＰＭ体内、外给药，均可抑制关节炎大鼠腹腔ＭΦ产生ＴＮＦα。２５ｍｇ／ｋｇ的ＲＰＭ预防和治疗给药，均可抑制关节炎大鼠的迟发型超敏（ＤＴＨ）反应。结果表明：ＲＰＭ对大鼠佐剂性关节炎的防治作用可能与其抑制ＴＮＦα的产生和ＤＴＨ反应有关。
In this paper, the effect of rapamycin (RPM) on adjuvant arthritis in rats was studied. It was demonstrated that intraperitoneal injection of RPM1.0 ~ 5.0 0 mg / kg (ip) can completely prevent Freund’s complete adjuvant (FCA ) Induced secondary swelling of joints in rats. Cyclosporin A (CsA) was better than RPM in the acute phase of FCA-induced acute swelling in rats, but the rebound occurred immediately after CsA withdrawal. For secondary swollen joints, RPM5 0mg / kgi p also showed a good therapeutic effect, and does not rebound. The crystal violet staining of L929 cells showed that the concentration of tumor necrosis factor α (TNFα) produced by peritoneal macrophages (MΦ) in arthritic rats was significantly increased, and both the in vitro and in vivo administration of RPM could inhibit arthritis rats Peritoneal MΦ produce TNF α. Administration of 2.5 mg / kg RPM for prophylaxis and treatment inhibited the delayed-type hypersensitivity (DTH) response in arthritic rats. The results showed that: RPM on the prevention and treatment of adjuvant arthritis in rats may be related to its inhibition of TNF α production and DTH response.