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本文观察了肺炎克雷伯杆菌气道感染引起大鼠小气道炎症细胞和肺组织中TNF α含量动态变化。结果显示 ,肺炎克雷伯杆菌感染第 1天至第 1周 ,小气道壁中炎症细胞以中性粒细胞增多为主。感染第 2周至第 16周 ,小气道壁T淋巴细胞较对照组明显增多 (P <0 0 1) ,其阳性细胞百分率范围在 39%～ 67%。感染第 8周和第 16周 ,小气道壁CD4/CD8比值下降 ,分别为 0 5 7和 0 41,同时间对照组为 0 75和 0 78。树突状细胞 (S 10 0阳性细胞 )分布在小气道上皮 ,固有膜和粘膜下层。感染第 1周至第 8周 ,肺组织中TNF α含量明显升高 (P <0 0 1)。因此 ,在克雷伯杆菌感染引起的小气道炎症中 ,小气道壁T淋巴细胞 ,尤其CD8T细胞浸润值得重视。同时 ,肺组织中TNF α含量增高可能是T淋巴细胞在肺内积聚和数量增多机制之一。
In this paper, we observed the dynamic changes of TNFα in rat airway inflammation cells and lung tissue induced by Klebsiella pneumoniae airway infection. The results showed that in the first day to the first week of infection with Klebsiella pneumoniae, the inflammatory cells in the small airway wall mainly consisted of neutrophils. From the second week to the 16th week of infection, T lymphocytes in the small airway wall increased significantly (P <0.01), and the percentage of positive cells ranged from 39% to 67%. The CD4 / CD8 ratio in the small airway wall decreased at week 8 and week 16, respectively, at 0 5 7 and 0 41, and at 0 75 and 0 78 days in the control group. Dendritic cells (S 10 0 positive cells) are located in the small airway epithelium, lamina propria, and submucosa. From the first week to the eighth week of infection, TNFα levels in lung tissue were significantly increased (P <0.01). Therefore, Klebsiella pneumoniae infection caused by small airway inflammation, small airway wall T lymphocytes, especially CD8 T cell infiltration worthy of attention. In the meantime, the increase of TNFα level in lung tissue may be one of the mechanisms of T lymphocyte accumulation in the lung and its quantity.