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目的:采用ISSR标记构建云南省栽培灯盏花DNA指纹图谱并进行遗传多样性分析。方法:从20对候选引物中筛选出7对引物对云南10个地方栽培灯盏花进行扩增,通过扩增带型的差异构建其DNA指纹图谱,利用Popgen32、NTSYS2软件进行遗传一致性系数和遗传多样分析。结果:7对引物在10份共扩增出48条谱带,其中33条具有多态性,占68.8%,表明各居群的遗传差异较大。10个地方种质资源被聚为2个大类,野生居群被聚为一类,栽培灯盏花聚为一类,其中栽培灯盏花有两大亚类。结论:通过构建DNA指纹图谱容易地把灯盏花种植资源相互区分鉴别出来,10个地方灯盏花种质资源遗传多样性丰富,栽培灯盏花与野生具有差异,栽培种质材料之间也有差异。
OBJECTIVE: To construct DNA fingerprinting of Erigeron breviscapus from Yunnan Province using ISSR markers and analyze its genetic diversity. Methods: Seventeen pairs of primers were screened from 20 pairs of primers for amplification of 10 cultivars of Erigeron breviscapus in Yunnan Province. DNA fingerprinting was constructed by the difference of amplification bands. The genetic identity and inheritance were calculated using Popgen32 and NTSYS2 software Diverse analysis. Results: A total of 48 bands were amplified by 7 pairs of primers from 7 pairs of primers, of which 33 were polymorphic, accounting for 68.8% of the total, indicating a large genetic difference among populations. 10 local germplasm resources are grouped into two major categories, wild populations are grouped together, cultivated Breviscapus clustered into one category, of which there are two major cultivars of Breviscapus. Conclusion: It is easy to differentiate the resources of Erigeron breviscapus by constructing DNA fingerprinting. The germplasm resources of Erigeron breviscapi in 10 places are rich in genetic diversity. There are differences between cultivated Erigeron breviscapus and wild and there are also differences between cultivated germplasm materials.