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为了提高杀真菌素链霉菌菌株AL-04的发酵滤液对植物病原真菌的抑菌活性,采用单因素试验和均匀试验设计,对其发酵条件和培养基成分进行了研究.结果表明,菌株AL-04产抗生素的最适发酵培养基为:黄豆粉34.0g,鱼粉12.0g,蔗糖2.0g,可溶性淀粉6.0g,NaCl 2g,K2HPO40.5g,MgSO4.7H2O 0.1g,ZnSO40.01g,FeSO4.7H2O 0.01g,CaCO32g,蒸馏水1000mL;产抗生素的适宜发酵条件为:接种量10%,种子液菌龄36h,装液量50mL/300mL三角摇瓶,初始pH值7.2.在上述条件下,28℃、180r/min振荡培养7d,菌株AL-04的抑菌率比原始发酵培养基提高了13.6%,差异达到极显著水平(P<0.01).
In order to improve the antibacterial activity of fungicidal streptomyces strain AL-04 on phytopathogenic fungi, the fermentation conditions and culture medium composition were studied by single factor experiment and uniform design.The results showed that strain AL -04 optimum antibiotic fermentation medium: soybean meal 34.0g, fish meal 12.0g, sucrose 2.0g, soluble starch 6.0g, NaCl 2g, K2HPO40.5g, MgSO4.7H2O 0.1g, ZnSO40.01g, FeSO4.7H2O 0.01g, CaCO32g, distilled water 1000mL; antibiotic suitable fermentation conditions: inoculum 10%, the seedling age 36h, liquid volume 50mL / 300mL triangle flask, the initial pH 7.2. Under the above conditions, 28 ℃, The antibacterial activity of strain AL-04 increased by 13.6% compared with the original fermentation medium at 180r / min for 7 days. The difference was significant (P <0.01).