目的：观察神经酰胺对ＨＬ６０及中性粒细胞（ＰＭＮ）凋亡产生的影响，探讨其在粒细胞发生过程中可能的调节作用。方法：采用ＤＮＡ凝胶电泳，流式细胞术，光镜及ＲＴＰＣＲ等技术对细胞进行凋亡鉴定及相关基因ｂｃｌ２，ｃｍｙｃｍＲＮＡ表达分析。结果：神经酰胺（Ｃ２ｃｅｒａｍｉｄｅ），肿瘤坏死因子（ＴＮＦα）及长春新碱（Ｖｉｎｃｒｉｓｔｉｎｅ）均可不同程度地诱导ＨＬ６０及成熟ＰＭＮ产生凋亡，ＣＰＫ激活剂ＰＭＡ，Ｄｉａｃｙｌｇｌｙｃｅｒｏｌ无诱凋作用，并对ＴＮＦα，Ｃ２ｃｅｒａｍｉｄｅ及Ｖｉｎｃｒｉｓｔｉｎｅ诱导的凋亡有不同程度的抑制；上述药物刺激后ｂｃｌ２，ｃｍｙｃｍＲＮＡ表达在ＨＬ６０细胞中呈明显下调趋势，ｃｍｙｃｍＲＮＡ表达在ＰＭＮ中也呈轻度下调。结论：神经酰胺可作为ＨＬ６０、ＰＭＮ胞内介质参与凋亡信号的传导，且与ＣＰＫ途径及ｂｃｌ２，ｃｍｙｃ基因表达相关。 OBJECTIVE: To observe the effect of ceramide on the apoptosis of HL60 and neutrophil (PMN), and to explore the possible regulation of ceramide in granulocyte development. Methods: DNA gel electrophoresis, flow cytometry, light microscopy and RT  PCR techniques such as cell apoptosis identification and related genes bcl  2, c  mycmRNA expression analysis. Results: C2ceramide, TNFα and Vincristine all induced apoptosis in HL60 and mature PMN to some extent, while PMA and Diacylglycerol did not induce apoptosis. And the apoptosis induced by TNFα, C2ceramide and Vincristine were inhibited to varying degrees; the expression of bcl2 and cmycmRNA in HL60 cells was significantly downregulated after the above drug stimulation, and cmycmRNA was also expressed in PMN Showed a slight decrease. Conclusion: Ceramide can be used as HL60, PMN intracellular mediators involved in the signal transduction of apoptosis, and CPK pathway and bcl  2, c  myc gene expression.