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目的动态观察细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31疫苗免疫小鼠后脾细胞亚群的变化。方法将5×108克隆形成单位(CFU)和5×105CFU疫苗分别采用口服灌胃和鼻腔内接种免疫BALB/c鼠,在免疫后0、2、4、6、8、10、12、14、16、18和20周各剖杀4只小鼠,取脾,分离脾细胞,用流式细胞术(FCM)检测免疫小鼠脾CD4+和CD8+T细胞亚群的百分比,同时设双歧杆菌液体培养基(MRS)对照。结果口服灌胃组的脾CD4+T细胞亚群在免疫后4~10周升高,免疫后6周达最高水平,CD8+T细胞亚群无明显变化;鼻腔内接种组脾CD4+T细胞亚群在免疫后4~8周升高,免疫后6周达最高水平,CD8+T细胞亚群无明显变化。结论 CD4+T细胞亚群在细粒棘球绦虫重组Bb-Eg95-EgA31疫苗诱导的小鼠免疫应答中起关键作用。
Objective To observe the changes of splenocyte subsets in mice immunized with recombinant Bb-Egg-EgA31 vaccine of Echinococcus granulosus. Methods BALB / c mice were immunized with 5 × 108 clonogenic unit (CFU) and 5 × 105 CFU vaccine by oral gavage and intranasal inoculation, respectively. After immunization for 0,2,4,6,8,10,12,14, Four mice were killed on the 16th, 18th and 20th week respectively. Spleen cells were isolated and splenocytes were isolated. The percentage of splenic CD4 + and CD8 + T lymphocyte subsets in the immunized mice was determined by flow cytometry (FCM) Liquid Medium (MRS) Control. Results The levels of splenic CD4 + T cells in oral gavage group increased at 4 to 10 weeks after immunization and reached the highest level at 6 weeks after immunization. There was no significant change in CD8 + T cell subsets; Subgroups increased at 4 to 8 weeks after immunization, reaching the highest level at 6 weeks after immunization. There was no significant change in CD8 + T cell subsets. Conclusion CD4 + T cell subsets play a key role in the immune response induced by recombinant Bb-Eg95-EgA31 vaccine of mice with Echinococcus granulosus.