采用显微镜计数法、荧光分光光度法、叶绿素a含量测定法、可见分光光度法对利玛原甲藻(Prorocentrum lima)指数生长期不同浓度的培养液进行细胞密度测定。结果表明,后3种细胞计数方法与显微镜计数法相比较,均有较好的线性关系;同时对不同生长期的利玛原甲藻培养液进行测定,结果表明,4种方法的生长曲线一致性良好。比较分析发现,可见分光光度法适用于不同细胞密度范围的测定,操作简单、耗时短、不需要破坏样品、适于批量操作,可确定为便捷、有效、可行的利玛原甲藻细胞计数法。 The cell density was determined by microscope counting, fluorescence spectrophotometry and chlorophyll a content determination, and spectrophotometry with different concentration of culture medium of Prorocentrum lima exponential phase. The results showed that there was a good linear relationship between the latter three kinds of cell counting methods and the microscope counting method. Meanwhile, the growth of Prorocentrum lima algae culture medium at different growth stages was measured. The results showed that the growth curve consistency of the four methods good. Comparative analysis showed that visible spectrophotometry was suitable for the determination of different cell density range, simple operation, time-consuming, without destroying the sample, suitable for batch operation, can be determined to be convenient, effective and feasible Lypriopsis cell count law.