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人精子与去透明带金黄地鼠卵受精，在受精后异合卵培养阶段，用终浓度分别为２０μｇ／ｍｌ、４０μｇ／ｍｌ、６０μｇ／ｍｌ的间接诱变剂环磷酰胺进行处理，继而制备精子染色体进行核型分析。得到环磷酰胺诱发的染色体畸变精子率在上述剂量组依次为３３％、５０％、３３％；断裂均数依次为０．５４、１．９４和０７２。与空白对照组比较，差异具有显著性（Ｐ＜０．０５，Ｐ＜００１）。本研究结果表明，用人精子／金黄地鼠异合卵为靶细胞，能够检出间接诱变剂的诱变性。异合卵内源性代谢活化体系的发现和应用在哺乳动物配子的毒理遗传研究中具有重要意义。
Human spermatozoa and golden hamster ovums were removed from the zona pellucida and were treated with cyclophosphamide, an indirect mutagen at final concentrations of 20μg / ml, 40μg / ml and 60μg / ml, respectively Sperm chromosome karyotype analysis. Cyclophosphamide-induced chromosomal aberration sperm rate in the above-mentioned dose group was 33%, 50%, 33%; fracture were followed by 0.54,1.94 and 0.72. Compared with the blank control group, the difference was significant (P <0.05, P <001). The results of this study indicate that the mutagenicity of indirect mutagens can be detected by using human sperm / Hamster as target cells. The discovery and application of heterologous oocyte endogenous metabolic activation system is of great significance in the study of toxicological genetics of gametes in mammals.