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目的建立电感耦合等离子体-质谱(ICP-MS)法测定人全血、血清和尿中铟的方法。方法全血、血清和尿样均用质量分数为0.01%曲拉通X-100+质量分数为0.50%硝酸溶液10倍稀释,以铑标准溶液作为内标,采用ICP-MS法测定铟水平。结果使用正常人全血、血清、尿样配制的工作曲线与标准曲线的定量结果差异无统计学意义(P>0.05)。本方法测定全血、血清和尿中铟的线性范围为0.000~20.000μg/L,相关系数均>0.999,检出限为0.144μg/L;全血、血清和尿样的加标回收率分别为87.90%~95.92%、91.50%~94.20%和90.40%~96.57%,批内相对标准偏差(RSD)分别为3.81%~7.05%、3.75%~5.90%和4.31%~6.62%;批间RSD分别为2.90%~7.10%、3.80%~5.92%和4.16%~5.94%;样品在-20℃至少可保存14 d。对职业性铟接触工人(接触组,135人次)全血、血清和尿样进行检测,17人次全血和血清中均检出铟,检出率为1.26%;对照组工人(120人次)全血、血清、尿样和接触组工人尿样均无检出铟。结论本方法操作简单,具有较好的准确度和精密度,可对生物样品中的铟进行准确的定量分析。
Objective To establish a method for the determination of indium in human whole blood, serum and urine by inductively coupled plasma-mass spectrometry (ICP-MS). Methods Whole blood, serum and urine samples were all diluted 10-fold with 0.01% Triton X-100 + mass fraction of 0.50% nitric acid solution and rhodium standard solution as internal standard, and the indium level was determined by ICP-MS. Results There was no significant difference in the quantitative results between the working curve and the standard curve of normal human whole blood, serum and urine samples (P> 0.05). The linear range of this method for the determination of indium in whole blood, serum and urine was 0.000 ~ 20.000μg / L with the correlation coefficient of 0.999 and the detection limit of 0.144μg / L. The spiked recoveries of whole blood, serum and urine were Were 87.90% ~ 95.92%, 91.50% ~ 94.20% and 90.40% ~ 96.57%, respectively, and the relative standard deviations (RSDs) were 3.81% ~ 7.05%, 3.75% ~ 5.90% and 4.31% ~ 6.62% Respectively, from 2.90% to 7.10%, 3.80% to 5.92% and 4.16% to 5.94%. The samples can be stored for at least 14 days at -20 ℃. The detection of whole blood, serum and urine in occupational Indium exposure workers (135 contact groups) detected indium in 17 human blood and serum, with a detection rate of 1.26%. Control workers (120 persons) Indium was not detected in urine samples from blood, serum, urine samples and contact group workers. Conclusion The method is simple, accurate and accurate, and can accurately quantitatively analyze indium in biological samples.