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目的比较基因芯片法和培养法在细菌性痢疾诊断中的临床价值。方法采集临床诊断为细菌性痢疾的患者脓血黏液便,应用基因芯片技术进行病原菌的检测,并将结果与传统的培养法进行比较。结果 108例患者中,基因芯片法检测出痢疾杆菌37例,沙门菌13例,大肠埃希菌2例,病原菌检出率为48.1%;培养法检测出痢疾杆菌24例,沙门菌5例,病原菌检出率为26.9%;基因芯片法和培养法对病原菌、痢疾杆菌、沙门菌的检出率差异均有统计学意义(P<0.05)。培养法患者的基因芯片法均为阳性。培养法平均检测时间为48 h~72 h,基因芯片法平均检测时间为4.5 h。结论基因芯片技术对细菌性痢疾病原菌的检出具有快速、敏感的特点,可替代传统的培养法,在细菌性痢疾的早期诊断和治疗中具有重要的临床价值。
Objective To compare the clinical value of gene chip and culture in the diagnosis of bacillary dysentery. Methods The patients with clinical diagnosis of bacillary dysentery were collected for pus and mucus. The gene chips were used to detect the pathogens, and the results were compared with the traditional culture method. Results Among the 108 patients, 37 cases of Shigella dysenteriae, 13 cases of Salmonella and 2 cases of Escherichia coli were detected by gene chip method, the detection rate of pathogenic bacteria was 48.1%; 24 cases of Shigella dysenteriae, 5 cases of Salmonella, The detection rate of pathogenic bacteria was 26.9%. The detection rates of pathogen, dysentery bacillus and salmonella by gene chip method and culture method were all significantly different (P <0.05). Gene therapy in patients with gene chips were positive. The average detection time of culture method was 48 h ~ 72 h, and the average detection time by gene chip method was 4.5 h. Conclusion Gene chip technology has rapid and sensitive detection of bacterial dysentery pathogens, which can replace the traditional culture method and has important clinical value in the early diagnosis and treatment of bacterial dysentery.