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采用反转录-ＰＣＲ方法，分节段扩增汉坦病毒Ａ９株Ｍ基因片段ｃＤＮＡ，并克隆入ｐＧＥＭＴ载体中，用双脱氧末端终止法直接测定序列。结果表明，Ａ９株Ｍ片段ｃＤＮＡ由３６１８个碱基组成，编码１１３５个氨基酸， 核苷酸序列及由其推导的氨基酸序列同７６／１１８株的同源性分别为９４．３３％和９７．８０％，说明汉坦病毒Ａ９株与７６／１１８株在糖蛋白的结构上高度保守。同７６／１１８株比较，在３’末端非编码区变异极大，变异率为２０％，且多了２个核苷酸。Ａ９株Ｍ基因片段核苷酸序列的获得，为利用该ｃＤＮＡ进行基因工程疫苗的研制创造了条件。
The reverse transcriptase - polymerase chain reaction (PCR) was used to amplify the cDNA of M gene of Hantavirus strain A9 by segmented amplification and cloned into pGEMT vector. The sequence was directly determined by dideoxy terminator method. The results showed that the M segment cDNA of A9 strain consisted of 3618 bases and encoded 1135 amino acids. The homology of the deduced amino acid sequence with that of 76/118 was 94.33% and 97.80 %, Indicating that the Hantavirus strains A9 and 76/118 are highly conserved in glycoprotein structure. Compared with the 76/118 strain, there is a great variation in the 3’-end non-coding region with a mutation rate of 20% and more than two nucleotides. A9 strain M gene fragment nucleotide sequence obtained for the use of the cDNA genetically engineered vaccine to create the conditions.