鸡蛋清中残留的金霉素(CTC)易进入人体内蓄积,从而对人体的健康产生损害。研究表明,银胶能作为表面基底来修饰金霉素与Eu(III)的配合物,进而使配合物的荧光强度得到显著性的增强。本研究应用银胶表面增强Eu(III)的荧光来检测鸡蛋清中金霉素的残留量,分别分析了不同胶体、银胶的加入量、Eu(III)的加入量、反应时间等因素对荧光强度的影响,并确定了最佳的实验条件,在最佳条件下建立了以617 nm处的荧光特征峰强度(Y)与鸡蛋清中金霉素含量(X)之间的标定曲线。结果表明:鸡蛋清中金霉素浓度范围在1.5~29.5 mg/L时,鸡蛋清中金霉素含量与荧光强度之间呈现良好的线性关系,线性方程为Y=5.2265X+24.033,决定系数R2为0.9043,实测值与预测值之间的R2=0.9182。可见用银胶表面增强Eu(III)的荧光来快速检测鸡蛋清中金霉素的残留量是可行的。 Chrysanthemum residues in egg white (CTC) easy to accumulate in the human body, thus causing damage to human health. The results showed that silver colloids could be used as the surface substrate to modify the complexes of chlortetracycline with Eu (III), which further enhanced the fluorescence intensity of the complexes. In this study, we detected the residual amount of chlortetracycline in the egg white by the fluorescence of Eu (III) on the surface of silver colloids. The effects of the addition of different colloids and silver colloids, the addition amount of Eu (III) and the reaction time Fluorescence intensity and determine the best experimental conditions, under the best conditions to establish a calibration curve between the fluorescence intensity peak at 617 nm (Y) and choromycin content in egg white (X). The results showed that there was a good linear relationship between the concentration of chlortetracycline in chicken egg white and the fluorescence intensity when the concentration of chlortetracycline was 1.5 ~ 29.5 mg / L. The linear equation was Y = 5.2265X + 24.033. The coefficient of determination R2 is 0.9043, R2 = 0.9182 between the measured value and the predicted value. It can be seen that it is feasible to detect the residual amount of chlortetracycline in egg white by the fluorescence of Eu (III) on the surface of silver gel.