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目的 研究水飞蓟素和山莨菪碱拮抗糖基化产物和抑制牛视网膜毛细血管周细胞增生的作用。方法 采用MTT比色测定结合3H -TdR掺入 ,观察水飞蓟素和山莨菪碱对早期糖基化白蛋白产物 (earlyglycosylationproductsofalbumin ,EGPA)和糖基化终产物 (advancedglycosy lationendproductsofalbumin ,AGEPA)培养下的周细胞增生和DNA合成的影响。结果 在EGPA和AGEPA培养下 ,MTT测定的A值水飞蓟素组分别为 0 491 9± 0 0 6 2 0和 0 40 0 3± 0 0 6 92 ,与对照组 (0 46 1 9± 0 0 5 39和 0 3884±0 0 82 6 )比较分别为P <0 0 1和P >0 0 5 ;山莨菪碱组分别为0 482 6± 0 0 5 36和 0 390 7± 0 0 748,与对照组比较P >0 0 5 ;3H -TdR掺入量 (Cpm) :水飞蓟素组分别为 44 76 7 6 7± 1 6 33 2 9和 40 0 1 5 44± 890 78,与对照组 (4 1 82 5 5 4± 1 45 2 6 7和 382 37 0 0±1 2 91 2 5 )比较P <0 0 1和P >0 0 5 ;山莨菪碱组分别为 41 75 1 2 2± 1 2 91 2 5和 390 6 7 6 7± 95 2 1 9,与对照组比较P >0 0 5。结论 山莨菪碱能有效地拮抗EGPA培养下的周细胞增生并抑制DNA的合成 ,但对AGEPA培养下周细胞的上述改变无明显保护作用 ;山莨菪碱对EGPA和AGEPA所致的周细胞增生的抑制和DNA合成的减少?
Objective To study the effects of silymarin and anisodamine on the glycosylation products and the inhibition of proliferation of bovine retinal capillary pericytes. Methods MTT colorimetric assay combined with 3H-TdR incorporation was used to observe the proliferation of pericytes cultured by silymarin and anisodamine against early glycosylated albumin (EGPA) and advanced glycosylation endproducts of albumin (AGEPA) cultures. And the effects of DNA synthesis. Results In the EGPA and AGEPA cultures, the A value of silymarin measured by MTT was 0 491 9 ± 0 0 6 0 and 0 40 0 3 ± 0 0 6 92 , respectively, and the control group (0 46 1 9 ± 0 0 5 39). Compared with 0 3884±0 0 6 6 6), P 0 01 and P 0 05 respectively; in anisodamine group, they were 0 4 6 6 6 0 05 36 and 0 3 0 7 7 0 0 748, respectively. Comparing P >0 0 5 ; 3H -TdR incorporation (Cpm): Silymarin was 44 76 7 6 7 ± 1 6 33 2 9 and 40 0 1 5 44 ± 890 78, respectively, and the control group (4 1 82 5 5 4± 1 45 2 6 7 and 382 37 0 0±1 2 91 2 5) Comparing P <0 0 1 and P >0 0 5; Anisodamine group was 41 75 1 2 2 ± 1 2 91 2 5, respectively. And 390 6 7 6 7± 95 2 1 9 compared with the control group P >0 0 5. Conclusion Anisodamine can effectively antagonize the proliferation of pericytes and inhibit the synthesis of DNA under the culture of EGPA, but it has no obvious protective effect on the above changes of AGEPA cultured pericytes; the anisodamine has an effect on the proliferation of pericytes caused by EGPA and AGEPA. Inhibition and reduction of DNA synthesis?