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目的在人大肠杆菌中表达人ＣＤ８０胞外区蛋白质。方法采用ＰＣＲ扩增人ＣＤ８０胞外区基因ｃＤＮＡ，先后经Ｋｌｅｎｏｗ和ＨｉｎｄⅢ酶切并纯化，再定向克隆至高效表达载体ｐＫｐＬ－３ａ中，构建成ｐＫｐＬ－ＣＤ８０。转化大肠杆菌ｐｏｐ２１３６后，温度诱导表达，再用Ｗｅｓｔｅｒｎｂｌｏｔ鉴定之并检测其活性。结果转化菌表达出分子量为２４０００的蛋白质，免疫学鉴定为ＣＤ８０，并证实有一定生物学活性。结论大肠杆菌中可以成功表达出具有生物学活性的ＣＤ８０，为进一步研究和开发ＣＤ８０打下了基础。
Objective To express human CD80 extracellular domain protein in human E.coli. Methods The cDNA of extracellular region of human CD80 was amplified by PCR, digested and purified with Klenow and Hind Ⅲ, and then cloned into pKpL-3a expression vector pKpL-3a to construct pKpL-CD80. After transformed into E. coli pop2136, the expression was induced by temperature, and then identified by Western blot and tested for its activity. Results The transformed bacteria expressed a protein of 24000 in molecular weight and immunologically identified as CD80, and confirmed that it has certain biological activity. CONCLUSION: The bioactive CD80 can be successfully expressed in E. coli, laying a foundation for further research and development of CD80.