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以一套用籼稻品种9311为受体、粳稻品种日本晴为供体构建的128个染色体片段代换系为材料,按随机区组实验设计种植,于成熟后考查代换系粒长、粒宽性状,利用多元回归分析方法,结合Bin图谱,鉴定出6个与粒长相关的QTL、2个与粒宽相关的QTL。其中,qGL3.1被定位在水稻第3染色体的5 792 954bp区间内;qGL3.2被定位在第3染色体的917 878bp区间内;qGL8.1被定位在第8染色体的889 543bp区间内;qGL8.2被定位在第8染色体的208 614bp区间内;qGL9.1被定位在第9染色体的1 149 685bp区间内;qGL11.1被定位在第11染色体的3 184 760bp区间内;qGW1.1被定位在第1染色体的200 070bp区间内;qGW5.1被定位在第5染色体的704 905bp区间内。上述QTL的准确定位,为进一步精细定位及克隆相应QTL和开展水稻粒型分子育种奠定了基础。
A set of 128 chromosomal segment substitution lines constructed with indica variety 9311 as acceptor and japonica variety Nipponbare as donor were planted in randomized block experiments. After maturity, the length of substitution lines, grain-width traits, Using multivariate regression analysis and Bin map, six QTLs related to grain length and two QTLs related to grain width were identified. Among them, qGL3.1 was located in the interval of 5 792 954 bp on chromosome 3 of rice; qGL3.2 was located in interval 917 878 bp of chromosome 3; qGL8.1 was located in interval 889 543 bp of chromosome 8; qGL8 .2 was mapped in the interval of 208 614 bp on chromosome 8; qGL9.1 was located in the interval of 1 149 685 bp on chromosome 9; qGL11.1 was mapped on interval 3 184 760 bp of chromosome 11; qGW1.1 was Located in the 200 070bp interval of chromosome 1; qGW5.1 was located in the 704 905bp interval of chromosome 5. The accurate location of these QTLs laid the foundation for further fine mapping and cloning of QTLs and breeding of rice grain type molecular markers.