论文部分内容阅读
茭白肉质茎的形成是菰(Zizania latifolia)与菰黑粉菌(Ustilago esculenta)互作的结果,茭白植株与菰黑粉菌间必然存在一系列的免疫防御反应。研究激活抗性基因(activated disease resistance 1,ADR1)在茭白生长发育中的表达模式有助于探讨茭白的孕茭机理。本研究克隆获得茭白ZlADR1基因(Gen Bank登录号:KP 729625.1),其c DNA全长1 164 bp,编码387个氨基酸,基因组序列包含有2个内含子,氨基酸序列分析发现ZlADR1含有Kinase2a、Kinase3a、RNBS-C、HD等结构域,属于CC-NBS-LRR类抗病相关基因;ZlADR1与野生水稻(Oryza brachyantha)中同源基因序列相似性最高。分析ZlADR1基因在茭白茎部发育期间、不同表型及不同部位间的表达发现:ZlADR1基因在茭白茎部膨大发育前上调表达,可能与此时茎部菰黑粉菌菌丝的大量生长相关;茭白茎部和叶片中均有ZlADR1基因表达,但有菰黑粉菌菌丝大量生长分布的茎部表达量显著高于叶片(P<0.05);此外,茭白膨大初期茎部ZlADR1基因表达量显著高于(P<0.05)灰茭及雄茭,而灰茭和雄茭之间无显著差异。由此可见,菰黑粉菌菌丝的生长及分布能够诱导调节ZlADR1基因表达,茎部菰黑粉菌菌丝的活体营养生长状态及分布与ZlADR1基因表达关系密切。本研究初步揭示了在茭白孕茭中ZlADR1基因的表达响应,为探讨茭白孕茭机理研究提供了理论依据。
The formation of fleshy stems is a result of the interaction between Zizania latifolia and Ustilago esculenta, and a series of immune defensive responses inevitably exist between the plants of Ficus andyzia. Studying the expression pattern of activated receptor resistance 1 (ADR1) in development and development of whitefly is helpful to explore the mechanism of whitefly pregnancy. In the present study, the ZlADR1 gene (GenBank accession number: KP729625.1) was cloned and the full-length cDNA was 1 164 bp in length and encoded 387 amino acids. The genomic sequence contained two introns. The amino acid sequence analysis revealed that ZlADR1 contains Kinase2a, Kinase3a , RNBS-C and HD, belong to the CC-NBS-LRR class of disease-related genes, and ZlADR1 has the highest similarity with the homologous gene sequences in wild rice (Oryza brachyantha). Analysis of ZlADR1 gene expression in different phenotypes and different sites during the development of Ziziphus hortensis showed that ZlADR1 gene was up-regulated before the enlargement and development of Ziziphus hongkai, which may be related to the large-scale growth of Zizyphus sp. The expression of ZlADR1 gene in stems and leaves of Zanthoxylum bungeanum was higher than that of the leaves (P <0.05). In addition, the expression of ZlADR1 gene in stems of Zanthoxylum bungeanum was significantly increased (P <0.05), while there was no significant difference between the gray goat and the male goat. Thus, the growth and distribution of black mold powdery mildew can induce the expression of ZlADR1 gene, and the live vegetative growth status and distribution of black mold powdery mildew in stems were closely related to the expression of ZlADR1 gene. This study initially revealed the expression of ZlADR1 gene in pomfret and provided a theoretical basis for the study on the mechanism of plicata.