论文部分内容阅读
目的 探讨大鼠甲状腺中是否存在促性腺激素释放激素受体 (GnRH R)及其细胞定位。方法收集 15例雄性SD大鼠甲状腺 ,分别制成石蜡切片和冰冻切片 ,采用免疫组织化学ABC法和原位杂交技术 ,确定GnRH R在其中的表达与定位。结果 大鼠甲状腺中 ,GnRH R呈较强的免疫反应阳性 ,阳性物质分布在胞质 ,胞核呈阴性。原位杂交也检测到较强的GnRH RmRNA阳性杂交信号 ,亦分布在胞质 ,胞核未见表达。结论大鼠甲状腺可以自身合成GnRH R。由此推断GnRH可能参与大鼠甲状腺功能的调节
Objective To investigate whether GnRH receptor (GnRHR) and its cellular localization exist in the thyroid of rats. Methods Fifteen male Sprague Dawley rats were collected and paraffin sections and frozen sections were obtained. Immunohistochemical ABC and in situ hybridization were used to determine the expression and localization of GnRH R. Results GnRH R was strongly immunoreactive in the thyroid gland of rats. The positive substances were distributed in the cytoplasm and the nuclei were negative. In situ hybridization also detected a strong GnRH RmRNA positive hybridization signal, also distributed in the cytoplasm, no expression in the nucleus. Conclusion The rat thyroid gnRH can synthesize itself. It is concluded that GnRH may be involved in the regulation of thyroid function in rats