Aim: This study aims to assess the infection risks of flashlight contamination in a stomatology hospital and compare the disinfection effectiveness of alcohol (75％) and disinfecting wipes.Background: The flashlight is a basic non-critical medical device in oral and maxillofacial surgery wards. Wounds are mostly found in oral cavities; therefore, reusable flashlights may be a potential source of nosocomial infections (NIs). However, the microbial flora present in flashlights used in hospitals has not yet been explored. Methods: This study investigated the microbial contamination of 41 flashlights used in a stomatology hospital in Guangzhou in March 2016. Results: Results indicated that 75.6％(31/41) of the flashlights had microbial contamination. Gram-positive bacteria accounted for 72.7％(24/33)of the microbial groups contaminating the flashlights, and Gram-negative bacteria (21.2％, 7/33), and fungi (6.1％, 2/33) constituted the remaining contaminants. The predominantly isolated species was Staphyloccus (66.7％, 22/33), especially Staphylococcus aureus (24.2％, 8/33). Approximately 77.3％(17/22) of the types of bacteria detected in the hands were same as those in the corresponding flashlights. Both the bacterial overstandard and S. aureus detection rates of doctors’ flashlights were higher than those of nurses’ flashlights (16/17 vs. 14/23, 7/17 vs. 1/23, respectively) (P < 0.05). Moreover, both disinfectants performed excellently, and their eligibility rates were not significantly different (17/17 vs. 14/14) (P>0.05). Conclusion: Flashlights are potential causes of NIs. Disinfecting flashlights could be an effective and practical infection control method.