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背景:已有较多的研究证明脐血细胞能向神经元样细胞分化,且成功的运用脐血治疗脑卒中及其他神经系统疾病的研究已有报道,能否应用脐血干细胞治疗神经变性疾病尚未可知。目的:探讨应用人脐血间充质干细胞治疗大鼠帕金森病的可行性及其机制。设计:随机对照实验研究。地点和对象:健康Sprague-Dawley大鼠18只,清洁级,体质量220～260g。脐血标本取自郑州大学第三附属医院妇产科,每个标本60～120mL。干预:制备6-羟多巴胺帕金森病偏侧大鼠模型,随机分为3组:①对照组(n=6)。②PBS组(n=6):在每只大鼠右侧纹状体移植10μL的PBS。③间充质干细胞组(n=6):将3×106个用BrdU标记的脐血间充质干细胞植入大鼠右侧纹状体,4周后用阿朴吗啡诱发旋转试验并用免疫组织化学检测植入细胞的存活情况及纹状体酪氨酸羟化酶阳性细胞。主要观察指标:①移植前后各组大鼠旋转圈数。②免疫组织化学染色结果。结果:脐血间充质干细胞在体内存活,与对照组相比,间充质干细胞移植组阿朴吗啡诱发的旋转行为犤每30钟大鼠旋转圈数为212±60比340±30犦显著改善(P<0.05),但右侧纹状体酪氨酸羟化酶阳性细胞数与对照组相比无显著性差异(P>0.05)。结论:人脐血间充质干细胞脑内移植能改善帕金森大鼠的行为缺陷,可作为治疗神经变性疾病的一种潜在细胞?
BACKGROUND: More studies have demonstrated that umbilical cord blood cells can differentiate into neuron-like cells, and the successful use of umbilical cord blood to treat stroke and other neurological diseases has been reported. Whether umbilical cord blood stem cells can be used in the treatment of neurodegenerative diseases has not been reported Can know. Objective: To investigate the feasibility and mechanism of human umbilical cord blood mesenchymal stem cells in the treatment of Parkinson’s disease in rats. Design: Randomized controlled trial. Location and objects: 18 healthy Sprague-Dawley rats, clean grade, body weight 220 ~ 260g. Cord blood samples taken from the Third Affiliated Hospital of Zhengzhou University Obstetrics and Gynecology, each specimen 60 ~ 120mL. Intervention: 6-hydroxydopamine Parkinson’s disease lateral rat model was prepared and randomly divided into three groups: ① control group (n = 6). PBS group (n = 6): 10 μL of PBS was transplanted to the right striatum of each rat. ③ MSCs group (n = 6): 3 × 106 Umbilical cord blood mesenchymal stem cells labeled with BrdU were implanted into the right striatum of rats. After 4 weeks, apomorphine-induced rotation test and immunohistochemistry Survival of implanted cells and striatal tyrosine hydroxylase positive cells were examined chemically. MAIN OUTCOME MEASURES: ① The number of rotations of rats in each group before and after transplantation. ② immunohistochemical staining results. Results: Umbilical cord blood mesenchymal stem cells survived in vivo. Compared with the control group, apomorphine-induced rotational behavior of rat mesenchymal stem cells was significantly (212 ± 60 vs. 340 ± 30 ±) rotations per 30 minutes (P <0.05). However, the number of tyrosine hydroxylase positive cells in the right striatum was not significantly different from that in the control group (P> 0.05). Conclusion: Human umbilical cord blood-derived mesenchymal stem cells transplantation can improve behavioral defects in Parkinson’s disease rats and may be used as a potential cell for the treatment of neurodegenerative diseases.