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目的:为预防和控制Hi相关疾病提供菌株分型资料。方法:分析比较4株从5岁以下幼儿中分离的荚膜型流感嗜血杆菌(Haemophilus influenzae,Hi)的血清分型、生物分型、脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)的结果。结果:4株荚膜型Hi中,2株Hib均为生物Ⅰ型;1株Hie为生物Ⅲ型;1株与a、f荚膜型抗血清有交叉凝集的Hia/f为生物Ⅰ型。4株PFGE型的相似度,为48.8%～58.5%,其中,2株Hib菌株之间的相似度仅为48.8%;MLST分型,2株Ⅰ型Hib同为ST-95型,1株生物Ⅲ型的Hie为ST-18型,1株生物Ⅰ型的Hia/f可能为新的ST型,等待提交验证。结论:4株荚膜型Hi彼此间遗传关联度小,提示人群中尚未形成优势荚膜型Hi的聚集。Hi当菌株生物型和血清型相同时,PFGE能较好地区分不同来源的菌株。
OBJECTIVE: To provide strain typing information for the prevention and control of Hi-related diseases. Methods: The serotypes, bio-typing, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence of four strains of Haemophilus influenzae (Hi) isolated from children under 5 years old were compared and analyzed. Type (MLST) results. Results: Among the four strains of capsular Hi, two strains of Hib were all biotype Ⅰ; one strain of Hie was biotype Ⅲ; one strain of Hia / f cross-aggregating with a and f capsular antisera was biotype Ⅰ. The similarity of four strains of PFGE was 48.8% -58.5%, among which, the similarity between two Hib strains was only 48.8%. MLST typing and two strains of type I Hib were the same as those of ST-95 and one Type III Hie is ST-18, and one biological type Hia / f may be a new type ST, pending submission of validation. CONCLUSIONS: Four capsular His have a low genetic association with each other, suggesting that no dominant Hippocampal Hi accumulation has been established in the population. Hi When the strains are of the same biotype and serotype, PFGE can better differentiate strains from different origins.