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目的研究氯化锂-毛果芸香碱致大鼠不同时期海马髓鞘损伤及少突胶质细胞系变化。方法健康成年雄性SD大鼠建立氯化锂-毛果芸香碱慢性癫模型,分为对照组、急性期组(致后24 h内)、潜伏期组(致后2周内)、慢性期组(致后2个月),各组(均n=4)。采用伊文思蓝法检测各组大鼠海马血脑屏障(BBB)通透性改变;免疫荧光染色法检测海马神经元数目、髓鞘碱性蛋白(MBP)表达水平、2’,3’-环核苷酸3’-磷酸二酯酶(CNPase)阳性成熟少突胶质细胞数目、未成熟少突胶质细胞标记物(O4)表达水平、硫酸软骨素蛋白多糖(NG2)阳性少突胶质祖细胞数目。结果大鼠致后,急性期组、潜伏期组和慢性期组大鼠海马各区域BBB通透性均较对照组增加(P<0.05),以急性期组增加最为显著。各组大鼠海马不同区域NeuN阳性神经元数目均较对照组显著下降(P<0.05)。潜伏期组和慢性期组海马各区域MBP表达水平及CNPase阳性细胞数目均显著低于对照组(P<0.05);各组海马CA1、Hilus区O4表达水平及NG2阳性细胞数目均较对照组显著增加(P<0.05),以潜伏期组增加最为明显。结论氯化锂-毛果芸香碱致大鼠海马区BBB通透性增加,未成熟少突胶质细胞和少突胶质祖细胞免疫荧光表达上调,髓鞘及成熟少突胶质细胞存在一定程度损伤。
Objective To investigate the changes of myelin damage and oligodendrocyte line in hippocampus of rats induced by lithium chloride-pilocarpine-induced injury in rats. Methods Chronic epileptic model of lithium chloride-pilocarpine was established in healthy male Sprague-Dawley rats. The rats were divided into four groups: control group, acute phase group (within 24 h), latent phase group (within 2 weeks after hysteria), chronic phase group After 2 months), each group (n = 4). Evans blue method was used to detect the changes of BBB permeability in hippocampus of rats in each group. The number of hippocampal neurons, expression of MBP, 2 ’, 3’- The number of nucleoside 3’-phosphodiesterase (CNPase) -positive mature oligodendrocytes, the expression level of immature oligodendrocyte markers (O4), the expression of oligodendrocyte-like polysaccharide (NG2) oligodendrocyte Progenitor cell number. Results BBB permeability of hippocampus in acute phase group, latent phase group and chronic phase group were higher than that in control group (P <0.05), and the increase in acute phase group was the most significant. The number of NeuN positive neurons in different hippocampus in each group was significantly lower than that in control group (P <0.05). The expression of MBP and the number of CNPase positive cells in hippocampus of both latency group and chronic phase group were significantly lower than those in control group (P <0.05). The expression of O4 and the number of NG2 positive cells in hippocampal CA1 and Hilus area were significantly higher than those in control group (P <0.05), the most obvious increase in the latency group. Conclusion The permeability of BBB in hippocampus of rats induced by lithium chloride-pilocarpine is increased, the expression of immunofluorescence of immature oligodendrocyte and oligodendrocyte progenitor cells is increased, and myelin and mature oligodendrocytes are damaged to some extent .