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基于白蚁16S rRNA基因序列差异设计一对引物进行曲颚乳白蚁PCR鉴定,该引物可以与曲颚乳白蚁线粒体DNA进行PCR反应,特异性扩增约200bp目的DNA片断,成功构建以曲颚乳白蚁16S rRNA基因序列为基础的mtDNA-PCR鉴定体系。
A pair of primers was designed based on the 16S rRNA gene sequence of termites. PCR was performed on the mitochondrial DNA of C. sinensis, and the specific DNA fragment of about 200 bp was amplified by PCR. rRNA gene sequence based mtDNA-PCR identification system.