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为了解芝麻的耐旱性及获得与芝麻耐旱性相关的分子标记,在发芽期,采用不同浓度PEG 6000处理不同来源不同种皮颜色的10份芝麻品种,测定其形态特征和生理特征及各指标的差异显著性。结果表明,模拟芝麻干旱处理的最佳PEG6000浓度为15%;综合各项指标的主成分分析、最优回归方程分析及相关性分析表明,相对成苗率可以作为芝麻发芽期耐旱性鉴定的关键指标;利用上述方法对216份核心种质资源群体进行耐旱性鉴定,相对成苗率耐旱系数值位于12.15%~93.52%,平均为60.74%,变异系数为25.22,变异丰富且呈正态分布;资源群体的耐旱性指标值与608个多态性标记位点的关联分析,获得与芝麻发芽期耐旱性有显著关联的标记30个(P<0.05),解释率在1.99%~4.96%之间,平均2.84%。试验表明,相对成苗率是最适且最方便的耐旱性鉴定指标,适用于芝麻资源的耐旱性鉴定。
In order to understand the drought resistance of sesame and obtain the molecular markers related to the drought tolerance of sesame, 10 varieties of sesame seeds of different seed coat color were treated with PEG 6000 at different germination stages, and their morphological and physiological characteristics were measured Significant difference between the indicators. The results showed that the optimal concentration of PEG 6000 for simulating sesame was 15%. Principal component analysis, regression analysis and correlation analysis showed that the relative seedling emergence rate could be used to identify the drought tolerance of sesame during germination period Drought resistance of 216 core germplasm resources were identified by using the above method. The relative drought resistance of seedlings was 12.15% ~ 93.52% with an average of 60.74%, the coefficient of variation was 25.22, the variation was rich and positive 30 populations (P <0.05) were significantly correlated with the drought tolerance of sesame during the germination stage, with an interpretation rate of 1.99% ~ 4.96%, an average of 2.84%. The results showed that the relative seedlings rate was the most suitable and most convenient drought tolerance identification index, suitable for drought resistance identification of sesame resources.