In this research, 155 cotton introgressed lines from interspecific hybridization have been col- lected for the purpose of evaluating the effects of enhancement of new upland cotton germplasm by interspecific hybridization, screening for elite germ- plasm and improving cotton breeding. Through iden- tification of various agricultural traits, we found that different wild cotton species had different capacities in terms of fiber quality, disease resistance, tolerance of abiotic stress, pest resistance and so on. SSR molecular marker technology has been used to de- tect the exotic genetic elements in interspecific hy- brids, and 25 SSR specific loci that can be classified into two groups were found among 15 pairs of SSR primers. Results also showed that 8 exotic germ- plasm (Gossypium barbadense, G. arboreum and G. thurberi, etc) had genetic transmission toward upland cotton. A strategy of screening elite germplasm with exotic genes based on the molecular marker-assisted breeding techniques was suggested, and 18 lines with high-quality fiber and 4 lines with resistance to Fusanrium wilt and Verticillium wilt have been ob- tained. In this research, 155 cotton introgressed lines from interspecific hybridization have been col- lected for the purpose of evaluating the effects of enhancement of new upland cotton germplasm by interspecific hybridization, screening for elite germ- plasm and improving cotton breeding. various agricultural traits, we found that different wild cotton species had different capacities in terms of fiber quality, disease resistance, tolerance of abiotic stress, pest resistance and so on. SSR molecular marker technology has been used to de- tect the exotic genetic elements in interspecifichybrids, and 25 SSR specific loci that can be classified into two groups were found among 15 pairs of SSR primers. Results also showed that 8 that exotic germ-plasm (Gossypium barbadense, G. arboreum and G. thurberi, etc) had genetic strategy toward upland cotton. A strategy of screening elite germplasm with exotic genes based on the molecular marker-assisted breeding technique s was suggested, and 18 lines with high-quality fiber and 4 lines with resistance to Fusanrium wilt and Verticillium wilt have been ob- tained.