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目的探讨深圳市龙岗区感染性腹泻病人中沙门氏菌的感染状况及分子流行病学分析。方法 2010-2013年间从深圳市龙岗区人民医院感染性腹泻监测哨点,共采集腹泻病人粪便标本3 604份,按国标GB/T 4789.4-2013对样本进行分离鉴定,采用脉冲场凝胶电泳(PFGE)对沙门菌进行分子分型分析。结果 3 604份腹泻标本中,男女比例为1.25∶1,病例构成主要集中在0~6岁,所采样本中阳性数829例,分离出沙门菌142株,占感染性腹泻病原谱的17.13%(142/829),检出率最高的是8-9月占34.51%(49/142);血清分型结果表明该地区流行的沙门菌优势血清型为常见的肠炎和鼠伤寒沙门氏菌,PFGE带型表明肠炎沙门菌之间具有高度的同源性。结论深圳市龙岗区腹泻病例主要由沙门菌感染引起,肠炎沙门菌为常见的病原菌,其PFGE带型相似度高,来自同一克隆。
Objective To investigate the prevalence and molecular epidemiological analysis of Salmonella in patients with infectious diarrhea in Longgang District of Shenzhen City. Methods A total of 3 604 fecal samples from diarrhea patients were collected from the sentinel sites of infectious diarrhea in People’s Hospital of Longgang District from 2010 to 2013. The samples were isolated and identified according to the national standard GB / T 4789.4-2013. The pulsed-field gel electrophoresis PFGE) Salmonella molecular typing analysis. Results Among 3 604 samples of diarrhea, the ratio of male to female was 1.25:1. The cases were mainly composed of 0 to 6 years old. The number of positive samples was 829 and 142 strains of Salmonella were isolated, accounting for 17.13% (142/829). The highest detection rate was 34.51% (49/142) in August-September. The serotyping results showed that the predominant serotypes of Salmonella in this area were common enteritis and Salmonella typhimurium, PFGE band Type shows that Salmonella enteritidis has a high degree of homology. Conclusion The cases of diarrhea in Longgang District of Shenzhen City are mainly caused by the infection of Salmonella. Salmonella enteritidis is a common pathogen, and its PFGE band similarity is high and comes from the same clone.