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目的探讨脂蛋白酯酶在compound K调节血脂水平中的作用。方法 36只雄性SD大鼠随机分为6组,除对照组外均给予高脂饮食,对照组和模型组给予生理盐水腹腔注射,compound K给药组分别给予compound K 1、3、9 mg/kg腹腔注射,GGPP组同时给予compound K 3、9 mg/kg GGPP。4周后处死动物,取样测定血脂、肝素后脂蛋白酯酶活性、脂蛋白酯酶表达水平等;培养大鼠肝细胞株BRL,分别给予compound K 3、10、30μmol/L处理12 h后,检测脂蛋白酯酶表达水平。结果与模型组比较,compound K 3、9 mg/kg组的总胆固醇、低密度脂蛋白胆固醇、三酰甘油显著降低,高密度脂蛋白胆固醇、肝素后血清脂蛋白酯酶活性和脂蛋白酯酶表达量显著增高,差异有统计学意义(P<0.05),且呈剂量依赖性。体外细胞试验结果与动物实验一致。结论 compound K能够通过增加脂蛋白酯酶的表达和活性达到降低高脂饮食大鼠血清三酰甘油的作用,其机制与compound K激活肝X受体α相关。
Objective To investigate the role of lipoprotein lipase in the regulation of serum lipid by compound K. Methods Thirty-six male Sprague-Dawley rats were randomly divided into 6 groups: high-fat diet was given except the control group, and the control group and model group were given intraperitoneal injection of saline. The compound K group was given compound K 1,3,9 mg / kg intraperitoneal injection, GGPP group also given compound K 3, 9 mg / kg GGPP. After 4 weeks, the animals were sacrificed and blood lipids, lipoprotein lipase activity after heparin and lipoprotein lipase expression were determined by sampling. The rat hepatocyte BRL was cultured and treated with compound K 3,10 and 30μmol / L for 12 h, Detection of lipoprotein lipase expression level. Results Compared with model group, the levels of total cholesterol, low density lipoprotein cholesterol, and triglyceride in compound K 3, 9 mg / kg group were significantly lower than those in model group. High density lipoprotein cholesterol, heparin serum lipoprotein lipase activity and lipoprotein lipase The expression was significantly increased, the difference was statistically significant (P <0.05), and in a dose-dependent manner. In vitro cell test results and animal experiments. Conclusion Compound K can reduce the serum triglyceride level in lipoprotein-induced rats by increasing the expression and activity of lipoprotein lipase, which is related to the activation of liver X receptor α by compound K.