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目的 采用尾蚴攻击感染小鼠后免疫的方式 ,研究日本血吸虫未成熟卵可溶性抗原 (SIEA)诱导抗卵胚胎发育和抗虫免疫的效果。方法 感染日本血吸虫尾蚴之后的第 2天用 SIEA免疫小鼠 ,每周 1次 ,共 5次 ,于第 48天剖杀小鼠 ,统计肝脏各期虫卵数、雌虫子宫虫卵数及每鼠成虫数。结果 与对照组比较 ,SIEA免疫诱导显著抗卵胚胎发育效果 ,肝组织成熟卵数下降 2 7.5 % ,成熟卵比例下降 11.0 % ,但 SIEA未诱导显著抗虫和抗雌虫生殖效果。结论 采取在尾蚴感染之后免疫 ,SIEA能诱导显著抗卵胚胎发育 ,但比尾蚴感染小鼠之前免疫的抗卵胚胎发育效果低
OBJECTIVE: To study the effect of immature egg soluble antigen (SIEA) induced by Schistosoma japonicum on anti-egg development and insect-resistant immunity in mice infected with cercariae. Methods The mice were immunized with SIEA on the second day after infection with Schistosoma japonicum cercariae. Five times a week, mice were killed on the 48th day. The number of eggs in each stage of the liver, Mouse adult number. Results Compared with the control group, SIEA induced a significant anti-egg development effect. The number of mature eggs decreased by 7.57% and the percentage of mature eggs decreased by 11.0%. However, SIEA did not induce significant anti-insect and feminine reproductive effects. Conclusion SIEA can induce significant anti-egg embryo development after immunization after cercariae infection, but it has less effect on the development of anti-egg embryo than before immunization.