论文部分内容阅读
本文报告单克隆抗体嵌板对100例急性白血病(儿童急淋为主)的诊断价值。试验所用单克隆抗体为鼠抗体如 DA2、AL2等,还有抗免疫球蛋白 Ig~F、X~D、λ~F及抗末端脱氧核苷转移酶(TDT)等共11种。其中5种(DA2、AL2、WT1,MY9、抗 TDT)能作为第1线筛选,使90%的急性白血病可以分型;其余6种抗体,一般也不会遗漏几种少见的白血病表现型。由于抗体类型多,一种抗体标记失败不会严重影响整个表现型。对不能用免疫表现型分类的疑难病例,可分析免疫球蛋白μ基因重组加以分型。100例急性白血病中97例有明确的免疫表现型。血液学诊断肯定的79例中,76例被免疫分型证实;未获肯定的21例中有19例可用免疫表现型分类,而且当时被有关医院作为选择性治疗的基础,说明免疫表现
This article reports the monoclonal antibody diagnosis of 100 cases of acute leukemia (children with acute lymphoblastic) diagnostic value. The monoclonal antibodies used in the experiment were murine antibodies such as DA2 and AL2, as well as 11 antiimmunoglobulin Ig ~ F, X ~ D, λ ~ F and anti-terminal deoxynucleotidyl transferase (TDT). Five of these (DA2, AL2, WT1, MY9, anti-TDT) can be screened as Line 1 to allow 90% of acute leukemias to be typed; the remaining 6 antibodies generally do not miss several rare leukemia phenotypes. Because of the large number of antibodies, failure of one antibody label will not severely affect the entire phenotype. Difficult cases that can not be classified using immunophenotyping can be analyzed by immunoglobulin μ gene recombination to type. 97 cases of 100 cases of acute leukemia have a clear immune phenotype. Of the 79 hematologic diagnoses confirmed, 76 were confirmed by immunophenotyping; 19 of 21 unidentified immunophenotypes were classified as immunosuppressive and were used as the basis for selective treatment by the hospital concerned