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病毒学试验中经常需用特异性抗体。抗体来源一般多采取免疫动物血清,有时也采用免疫动物腹水。近年来我们在虫媒病毒研究中,利用 Ehrlich 腹水癌诱导免疫小鼠产生腹水作为抗体来源,取得较满意的结果。现报告如下。1 材料与方法免疫动物用毒株,均为近年我们从蜱中分离的待鉴定株。昆明种小白鼠购自哈尔滨医科大学动物学部。免疫途径采用腹腔注射0.5ml。免疫程序为间隔7～10天,注射4次:第1针为灭活病毒加福氏完全
Virological tests often require specific antibodies. Antibodies are generally taken more than the serum of immunized animals, and sometimes immunized animals with ascites. In recent years, we in the arbovirus study, the use of Ehrlich ascites carcinoma induced immune mice produce ascites as a source of antibodies, obtained more satisfactory results. The report is as follows. 1 Materials and Methods Immunized animals with strains, are in recent years we isolated from ticks to be identified strains. Kunming mice purchased from the Department of Zoology, Harbin Medical University. The immunization route was intraperitoneal injection of 0.5ml. Immune procedures for an interval of 7 to 10 days, 4 injections: the first needle for the inactivated virus plus Freunds complete