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目的:检测肾细胞癌(renal cell carcinoma,RCC)组织中波形蛋白(vimentin)的表达,研究其表达水平与肿瘤临床病理特征及临床转归的相关性。方法:收集行肾根治性切除术并经病理证实为肾细胞癌的103例病例(92例为透明细胞癌,11例为乳头状细胞癌),平均随访时间为51.2个月,通过Western Blot检测肿瘤标本中vimentin蛋白的表达情况,对肿瘤大小、分期、分级、转移及生存率等临床病理资料与vimentin表达水平的相关性行统计学分析。结果:肾癌中vimentin蛋白的表达水平与非肿瘤组织相比显著增加,并且与肿瘤大小(P=0.002)、转移(P<0.001)、pT分期(P<0.001)、分级(P<0.001)、存活率(95.8%vs72.7%,P=0.002)有相关性。单变量分析显示vimentin的表达水平对肾细胞癌发展及预后具有预测意义(P<0.05)。结论:本研究的结果显示vimentin的高表达与肾细胞癌的进展呈正相关,检测vimentin表达水平可用于肾癌分期、分级及预后判断。
OBJECTIVE: To detect the expression of vimentin in renal cell carcinoma (RCC) tissues and to study its correlation with the clinicopathological features and clinical outcome. Methods: A total of 103 cases of renal cell carcinoma (92 cases of clear cell carcinoma and 11 cases of papillary carcinoma) undergoing radical nephrectomy and confirmed by pathology were collected. The mean follow-up time was 51.2 months. Western Blot The expression of vimentin protein in tumor samples was analyzed statistically with the correlation between the clinicopathological data such as tumor size, staging, grading, metastasis and survival rate and vimentin expression level. Results: The expression of vimentin protein in renal cell carcinoma was significantly higher than that in non - tumor tissue (P <0.001), and was significantly correlated with tumor size (P = 0.002), metastasis , Survival rate (95.8% vs72.7%, P = 0.002). Univariate analysis showed that the expression of vimentin had a predictive value for the development and prognosis of renal cell carcinoma (P <0.05). Conclusion: The results of this study show that the high expression of vimentin is positively correlated with the progress of renal cell carcinoma, and the detection of vimentin expression level can be used for staging, grading and prognosis of renal cell carcinoma.