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[目的]观察搜风祛痰法对血管紧张素Ⅱ致体外培养人脐静脉内皮细胞(HUVECs)NF-KB mRNA及iN-OSmRNA表达的影响,阐明搜风祛痰法的中药调控NF-KBmRNA与iNOSmRNA在AS过程中可能性的作用机理。[方法]制备兔活心汤含药血清。采用酶消化法培养HUVECs2-3代用于实验,采用RT-PCR法测定内皮细胞NF-KBmR-NA及iNOS mRNA。随机分为5组:正常对照组,AngⅡ组,低浓度中药血清+AngⅡ组中浓度中药血清+AngⅡ组高浓度中药血清+AngⅡ组。[结果]活心汤可抑制AngⅡ导致的内皮细胞损伤,减少NF-KBmRNA及iNOSmRNA的表达。[结论]活心汤可抑制AngⅡ所致的HUVECs损伤,保护血管内皮功能。
[Objective] To observe the effect of wind-removing and expectorant method on the expression of NF-κB mRNA and iN-OS mRNA in cultured human umbilical vein endothelial cells (HUVECs) induced by angiotensin Ⅱ and to elucidate the relationship between the regulation of NF- Mechanism of action of iNOS mRNA in AS process. [Method] Preparation of Rabbit Heart Soup containing serum. Cultured HUVECs 2-3-3 by enzyme digestion were used in the experiment. The NF-κB mR-NA and iNOS mRNA in endothelial cells were determined by RT-PCR. The rats were randomly divided into 5 groups: normal control group, Ang Ⅱ group, low concentration Chinese medicine serum + Ang Ⅱ group, medium Chinese serum + Ang Ⅱ group, high concentration Chinese medicine serum + Ang Ⅱ group. [Results] Huoxintang can inhibit the endothelial cell injury induced by AngⅡ and reduce the expression of NF-κB mRNA and iNOS mRNA. [Conclusion] Huoxintang can inhibit the injury of HUVECs induced by Ang Ⅱ and protect the function of vascular endothelial cells.