腺病毒用于人类基因治疗有许多优点,亦有很高的潜在应用价值。但目前所用腺病毒有3方面的缺点:①插入容量小,限8kb以下;②载体病毒蛋白的表达可能引起被感染组织的炎症反应;③被转基因的表达时间短。为此作者发展了一种新的移去所有的病毒编码序列腺病毒载体,外源插入容量增大,同时不再表达病毒蛋白,从而消除了病毒蛋白引起的炎症反应,据作者介绍转染后表达时间也增长。 Adenovirus for human gene therapy has many advantages, but also a high potential value. However, adenovirus currently used has three shortcomings: ① small insertion capacity, limited to 8kb or less; ② the expression of the carrier virus protein may cause inflammation in infected tissues; ③ transgenics expression time is short. To this end, the authors have developed a new adenovirus vector that removes all viral coding sequences, increases the amount of exogenous insertion, and no longer expresses viral proteins, thereby eliminating the viral protein-induced inflammatory response. According to the authors, Expression time also increased.