目的：采用快速固相萃取反相高效液相色谱法测定全血环孢素Ａ。方法：全血用水溶血，用乙腈和水混合液沉淀蛋白后，用ＳＰＥ固相萃取柱萃取，５０％甲醇冲洗杂质，９５％甲醇洗脱，挥干，乙腈重组，正己烷进一步除去杂质，重组液上机。本法采用柱温为７０℃的Ｃ１８反相色谱柱，以乙腈∶甲醇∶水（３∶１∶１，Ｖ∶Ｖ）为流动相，检测波长２１０ｎｍ。结果：本法最低检出浓度为２０ｎｇ·ｍｌ－１。至少在４０～１５００ｎｇ·ｍｌ－１范围内呈线性。平均方法回收率１０２．９％，ＲＳＤ均在１０％以内。结论：本法灵敏，特异，既适合临床常规检测又适合ＣｓＡ的药动学研究。 OBJECTIVE: To determine cyclosporine A in whole blood by rapid solid phase extraction-reverse phase high performance liquid chromatography. Methods: The whole blood was hemololyzed and the protein was precipitated with a mixture of acetonitrile and water. The protein was extracted with SPE SPE cartridge, washed with 50% methanol, eluted with 95% methanol, evaporated and evaporated to dryness. Acetonitrile was used for recombination. Hexane was used to further remove impurities. Liquid machine. In this method, a C18 reversed-phase column with a column temperature of 70 ° C was used. The mobile phase was acetonitrile: methanol: water (3: 1: 1, V: V) Results: The minimum detectable concentration was 20 ng · ml-1. At least in the range of 40 ~ 1500ng · ml-1 linear. The average recovery rate of 102.9%, RSD are less than 10%. Conclusion: This method is sensitive and specific, which is suitable for both clinical routine examination and pharmacokinetic study of CsA.