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目的 :观察内皮素 (ET)、一氧化氮 (NO)在糖尿病肾病各期中的致病作用。方法 :应用放射免疫计数法、硝酸盐还原酶法、微柱层析法测定 6 4例糖尿病患者及 30例正常对照者的内皮素 (ET)、一氧化氮 (NO)、糖化血红蛋白(GHb A1 C)、胰岛素 (INS)、C肽 (CP)、空腹血糖 (FBG)、 2 4h尿白蛋白 (U AER)、α1 微球蛋白 (α1 - MG)等指标。糖尿病患者分为无糖尿病肾病组 (DM 组 )、早期糖尿病肾病组 (DM 组 )、临床糖尿病肾病组 (DM 组 )。应用均数±标准差、t检验、直线相关分析等统计学方法分析实验数据。结果 :糖尿病患者 ET明显高于对照组 ,DM 增高特别明显 (P<0 .0 1)。DM组及 DM 组 NO水平增高 ,与对照组比较无统计学意义 (P >0 .0 5 ) ,但 DM 组与对照组比较显著增高 (P>0 .0 1) ,DM 组较对照组显著降低 ,(P <0 .0 1)。结论 :在糖尿病肾病各期中 ET、NO均参与致病作用 ,在早期糖尿病肾病高滤过期与 NO显著升高密切相关 ,在临床糖尿病肾病期与 NO显著减少、 ET明显增高密切相关。
Objective: To observe the pathogenic role of endothelin (ET) and nitric oxide (NO) in various stages of diabetic nephropathy. Methods: The levels of endothelin (ET), nitric oxide (NO) and glycosylated hemoglobin (GHb A1) in 64 diabetic patients and 30 normal controls were measured by radioimmunoassay, nitrate reductase and microcolumn chromatography. C, INS, C peptide, FBG, UAER and α1 microglobulin (α1 - MG) were measured. Diabetic patients were divided into diabetic nephropathy group (DM group), early diabetic nephropathy group (DM group) and clinical diabetic nephropathy group (DM group). Application of mean ± standard deviation, t test, linear correlation analysis and other statistical methods to analyze the experimental data. Results: The ET in diabetic patients was significantly higher than that in the control group, especially in DM (P <0.01). The levels of NO in DM group and DM group were significantly higher than those in control group (P> 0.05), but DM group was significantly higher than that in control group (P <0.01) Decrease (P <0. 01). CONCLUSION: ET and NO are involved in pathogenicity during the stages of diabetic nephropathy. It is closely related to the marked increase of NO during the hyperfiltration stage of early stage of diabetic nephropathy and significantly decreased and significantly increased of ET in clinical stage of diabetic nephropathy.