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以滑子蘑菌丝体为试材,利用热水浸提法提取多糖,采用体外细胞试验、琼脂凹环法,检测了滑子蘑多糖的抗肿瘤、抑菌活性,并研究了多糖的保湿特性,以期为滑子蘑多糖的开发利用提供参考依据。结果表明:与空白试验组相比,滑子蘑多糖对小鼠巨噬细胞Ana-1的促增长率为48.65%,对胃癌细胞HGC-27的增殖抑制率为58.21%,对乳腺癌细胞BT-549的增殖抑制率为76.83%,对宫颈癌细胞Hela-229的增殖抑制率为63.57%;滑子蘑多糖对革兰氏阴性菌大肠杆菌、沙门氏菌及革兰氏阳性菌金黄色葡萄球菌均有抑制作用,且随着浓度的升高,抑制性增强;在30℃、RH 45%的恒温恒湿条件下,保湿性大小顺序为壳聚糖>透明质酸>山梨醇>滑子蘑多糖>甘油,在30℃、RH 80%的恒温恒湿条件下,保湿性大小顺序为透明质酸>山梨醇>壳聚糖>滑子蘑多糖>甘油,2种条件下滑子蘑多糖的保湿率都高于甘油,说明滑子蘑多糖有较好的保湿性。
The polysaccharides were extracted from hot-water extract by using Mycelia of Pleurotus ostreatus, and the antitumor and antibacterial activities of polysaccharides from Pleurotus ostreatus were tested by in vitro cell assay and agar concoction method. Characteristics, in order to provide a reference for the development and utilization of slider mushroom polysaccharides. The results showed that compared with the blank experimental group, the growth rate of Ana-1 of mouse macrophage was 48.65% and the inhibition rate of HGC-27 to gastric cancer cell was 58.21% -549, the inhibitory rate of proliferation was 76.83%, and the inhibitory rate of proliferation on Hela-229 cervical cancer cells was 63.57%. The inhibitory rate of S.pummelo polysaccharides against Gram-negative bacteria Escherichia coli, Salmonella and Gram-positive Staphylococcus aureus The inhibitory effect was enhanced with the increasing of the concentration. Under the conditions of constant temperature and humidity of 30 ℃ and RH 45%, the order of the moisture retention was chitosan, hyaluronic acid, sorbitol, > Glycerol, under the conditions of constant temperature and humidity of 30% and RH 80%, the order of the moisture retention is hyaluronic acid> sorbitol> chitosan> skid mushroom polysaccharide> glycerin, the moisture retention rate of skid mushroom polysaccharide under the two conditions Are higher than glycerol, indicating that slider mushroom polysaccharides have better moisture retention.