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本实验用敌枯双按不同剂量(1.0、0.2、0.05、0.01 mg/kg/d)配成不同浓度的溶液分别供4个剂量组的 SD 大鼠(每组30只)饮用,并设对照组(30只,仅饮用去离子水)。在第55周处死动物,每组随机选3～5只取甲状腺、肝脏作电镜观察。结果:4个剂量组大鼠甲状腺滤泡腔扩大,滤泡细胞变扁平,粗面内织网扩张呈囊状,线粒体肿胀,嵴变短而少,核膜内陷,异染色质凝集呈板或染色质边集;部分滤泡上皮细胞脱屑到滤泡腔内,可见畸形核、裸核及核碎裂。肝细胞线粒体呈“O”形,“C”形,粗面内织网呈囊状扩张,大滴脂肪变,核染色质边集,核均质化。实验表明敌枯双对甲状腺、肝组织均有毒性作用,超微结构改变程度与农药剂量呈正相关。
In this experiment, SD rats (30 rats in each group) were dosed with 4 doses of different concentrations (1.0, 0.2, 0.05, 0.01 mg / kg / d) Group (30, only drinking deionized water). At the 55th week, animals were sacrificed, and 3-5 randomly selected thyroid gland in each group. The liver was observed under electron microscope. Results: The thyroid follicles in 4 doses group expanded, the follicular cells became flattened, the intrastromal network expanded into cystic shape, the mitochondria became swollen, the crest became shorter and fewer, the nuclear membrane invaded and the heterochromatin aggregated into plate Or chromatin edge set; part of the follicular epithelial cells scaling to the follicular cavity, showing deformed nucleus, bare nucleus and nuclear fragmentation. The mitochondria of hepatocytes showed “O” shape, “C” shape, cystic dilatation in rough surface, steamed fatty drops, nuclear chromatin margination and nuclear homogenization. Experiments show that the enemy drown double pairs of thyroid, liver tissue have toxic effects, changes in the degree of ultrastructure and pesticide dose was positively correlated.