本文介绍一种测定黄连及其制剂中小檗碱含量的方法——采用薄层层析分离后刮取小檗碱斑点,洗脱后以重铬酸钾硫酸液作对照标准,于421nm波长处进行比色测定。此法不需小檗碱标准品,所需样品量少,相对误差小,平均回收率可达99％。 This article describes a method for the determination of berberine in Rhizoma Coptidis and its preparations. After the separation by thin layer chromatography, the berberine spots are scraped off. After elution, a potassium dichromate solution is used as a reference standard at a wavelength of 421 nm. Colorimetric determination. This method does not require berberine standards, the amount of sample required is small, the relative error is small, and the average recovery rate can reach 99%.