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目的：探讨电镜冷冻蚀刻技术在Ｄｕｃｈｅｎｎｅ 型肌营养不良症（ ＤＭＤ） 研究中的应用。方法：电镜观察ＤＭＤ 及正常对照的骨骼肌和红细胞的冷冻蚀刻样品。结果：ＤＭＤ 患者红细胞与肌细胞一样，其细胞膜中的蛋白颗粒显著减少。结论：①冷冻蚀刻技术有助于ＤＭＤ 的诊断，其细胞膜存在缺陷；②可取红细胞代替肌组织进行诊断。
Objective: To investigate the application of electron microscopy cryoetching in the study of Duchenne muscular dystrophy (DMD). Methods: Electron microscopy was used to observe the freeze-dried samples of skeletal muscle and erythrocytes from DMD and normal controls. Results: Erythrocytes in DMD patients, like muscle cells, showed a significant reduction of protein granules in the cell membrane. Conclusion: ① Cryo-etching technique is helpful for the diagnosis of DMD, and its cell membrane is defective; ② Red blood cells can be used instead of muscle tissue for diagnosis.