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目的观察靶向脂质体携载降钙素基因相关肽（ｃａｌ－ｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）对大鼠主动脉内皮剥脱的保护作用。方法血管条灌流；放射免疫测定环磷酸鸟苷（ｃＧＭＰ）和内皮素（ｅｎｄｏｔｈｅｌｉｎ，ＥＴ）。结果由静脉注射靶向脂质体（ｌｉｐｏｓｏｍｅ，Ｌｓ）携载ＣＧＲＰ制备液（ＲＧＤＳ－Ｌｓ－ＣＧＲＰ６．２５μｇ·ｋｇ－１）能显著改善血管对乙酰胆碱（Ａｃｈ）的舒张反应（Ｐ＜０．０１），提高血浆ＮＯ－２含量（Ｐ＜０．０５）及主动脉组织中ｃＧＭＰ的含量（Ｐ＜０．０１），降低血浆ＥＴ含量（Ｐ＜０．０１）；抑制平滑肌细胞的过度增殖（Ｐ＜０．０１）。治疗作用明显优于相同剂量单纯ＣＧＲＰ组。结论靶向脂质体携载ＣＧＲＰ对冠状动脉再狭窄的发生具有一定的治疗作用，对临床治疗冠状动脉再狭窄提供一种新的给药方法
Objective To observe the protective effects of targeting liposomes on calcitonin gene related peptide (CGRP) on aortic endothelium exfoliation in rats. Methods The vascular strips were perfused. Radioimmunoassay was used to determine cGMP and endothelin (ET). Results The vasodilatory response of blood vessels to acetylcholine (Ach) was significantly enhanced by lipopolysaccharide (LPS) injection of RGDS-Ls-CGRP (6.25 μg · kg-1) (P <0.05) and the content of cGMP in the aorta (P <0.01), and decrease the content of ET in the plasma (P <0.01), and inhibited the proliferation of smooth muscle cells P <0.01). Therapeutic effect was significantly better than the same dose of simple CGRP group. Conclusion Targeted liposome-loaded CGRP has a certain therapeutic effect on the occurrence of coronary artery restenosis and provides a new method for the clinical treatment of coronary artery restenosis